One-Pot Hydrolysis of Cellulose and Hemicellulose in Lignocellulosic Biomass Pretreated by NaOH-Freeze

Biomass wastes were NaOH-freeze pretreated for one-pot hydrolysis with yields increased by >214% for cellulose, >59% for hemicellulose, respectively. （对生物质废弃物进行一锅法水解，NaOH 冷冻预处理后，纤维素水解率增加>214%，半纤维素水解率增加>59%。）

Recently, Miss Tongchao Su (PhD student from China University of Science and Technology) supervised by Prof. Zhen FANG hydrolyzed cellulose and hemicellulose in lignocelluloses in one-pot. In their work, in order to achieve one-pot hydrolysis of cellulose and hemicellulose in lignocellulosic biomass, freeze pretreatment with 0−5 wt % NaOH (3 wt% was chosen as the best concentration) and subsequent microwave-assisted dilute sulfuric acid hydrolysis were applied to tropical plant wastes. Single factor optimization of hydrolysis of the pretreated bagasse was studied, and the best conditions (140 °C, 30 s, substrate/solvent ratio of 1/60 and 0.4 M H2SO4) were further applied to Jatropha and Plukenetia hulls. Under the best conditions, yields of sugars from cellulose (glucose) and hemicellulose (xylose, mannose, and arabinose) in the pretreated bagasse were 84.0% and 92.7%, enhanced by 273.4% and 59.3% as compared with untreated sample, respectively. For the pretreated Jatropha and Plukenetia hulls, yields of glucose and hemicellulose sugars were 85.7% and 93.3% (increased by 225.9% and 75.4%), and 85.0% and 92.9% (increased by 214.8% and 81.4%), respectively.

The results were published:

TC Su, Zhen Fang*, One-pot microwave-assisted hydrolysis of cellulose and hemicellulose in selected tropical plant wastes by NaOH-freeze pretreatment, ACS Sustainable Chemistry & Engineering, 5(6), 5166–5174 (2017).

一锅法水解 经NaOH 冷冻预处理后的生物质中纤维素和半纤维素

最近，苏同超同学（中国科学技术大学生命学院博士研究生）在方老师的指导下，对木质纤维素中的纤维素和半纤维素进行了一锅水解的研究。

为了实现木质纤维素生物质中的纤维素和半纤维素的同时水解，首先对生物质用0−5 wt% NaOH（3wt%被选为最佳浓度）冷冻预处理，随后进行微波稀酸水解。

对预处理后的甘蔗渣水解条件的优化进行了单因素实验研究，并利用所得的最佳条件（140 °C，30秒，生物质/溶剂比为1／60和0.4 M H2SO4）进一步用于小桐子和星油藤壳的水解。在最佳条件下，和未经处理的样品相比，预处理后的甘蔗渣纤维素糖产量（葡萄糖）和半纤维素（木糖、甘露糖、阿拉伯糖）糖产量，分别为84%和92.7%，提高了273.4%和59.3%。对预处理后的小桐子和星油藤壳，产生葡萄糖和半纤维素糖分别为85.7%和93.3%（分别增加225.9%、75.4%），85%和92.9%（分别增加了214.8%和81.4%）。

结果发表在ACS Sustainable Chemistry & Engineering: TC Su, Zhen Fang*, One-pot microwave-assisted hydrolysis of cellulose and hemicellulose in selected tropical plant wastes by NaOH-freeze pretreatment, ACS Sustainable Chemistry & Engineering, 5(6), 5166–5174 (2017).