In the saccharification of lignocellulosic materials, enzymatic hydrolysis is regarded as a green approach operating under mild reaction conditions with subsequent higher glucose yields. However, the hydrolysis rate can be declined by the presence of lignin, which acts as a physical barrier shielding cellulose from enzymatic attack. Lignin can also cause losses of enzymes (cellulases and β-glucosidases) through their binding to lignin, and deactivation of enzyme activities can occur from products arising from lignin degradation. In order to access the cellulose and reduce lignin before hydrolysis, some pretreatment methods can be developed by using white-rot fungi.