Biodiesel production via simultaneous esterification and transesterification of Periplaneta americana oil with liquid lipase Eversa® transform 2.0
Recently, PhD student Miss Jing-jing Guo supervised by Prof. Zhen Fang,collaborated with Prof. WN Ding (College of Science and Technology, Ningbo University) and YT Wang (College of Metallurgy and Energy, North China University of Science and Technology) published a research article in Renewable Energy about biodiesel production from high acid value oil with liquid lipase.
Undeveloped Periplaneta americana oil (acid value 38.32 mg KOH/g) was directly used for one-step production of biodiesel with lipase without acid-pretreatment step for the commercial alkaline process. Biodiesel was produced via simultaneous esterification and transesterification of Periplaneta americana oil in the presence of lipase Eversa® Transform 2.0 (ET2) (12 US$/kg) in solvent-free system. The maximum biodiesel yield of 98.63% was obtained under the optimized conditions of 32 °C, 8.5 wt.% lipase dosage, 8 h, 6.5/1 methanol/oil molar ratio, and 4 wt.% water. Lipase ET2 was recycled 6 times at > 89.52 % biodiesel yield. Biodiesel yield of 93.94 % was further achieved in a 1 L reactor with 15.08 g/kg lipase/biodiesel. Biodiesel cost was estimated as 589.3 US$/ton. Kinetics study gave activation energy of 24.50 kJ/mol with kinetic Michaelis constant of 1.19 mol/L. The physicochemical properties of biodiesel met both Chinese national and US ASTM standards that could be blended with petro-diesel to be applied in both countries. This study suggests that lipase could directly catalyze waste oils for the production of biodiesel at low temperature.
Related results were published in Renewable Energy:
JJ Guo, S Gao, J Yang, H Zhang, YT Wang*, WN Ding*, Zhen Fang*. Biodiesel production via simultaneous esterification and transesterification of Periplaneta americana oil with liquid lipase Eversa® transform 2.0. Renewable Energy (IF 9), 229 (2024), 120756. https://doi.org/10.1016/j.renene.2024.120756
Liquid lipase Eversa® Transform 2.0 catalyzed high acid value Periplaneta americana oil for biodiesel production with 98.63% yield and recycled 6 times at > 89.52% biodiesel yield. （液体脂肪酶催化高酸值美洲大蠊油制备生物柴油的产率为98.63%。酶回收6次，生物柴油产率仍可达 > 89.52%）。
液体脂肪酶Eversa® Transform 2.0催化美洲大蠊油同时进行酯化和酯交换反应制备生物柴油
近期，博士生郭静静同学在方真教授的指导下，与宁波大学科技学院丁沃娜教授和华北理工大学冶金与能源学院王一同教授合作，在国际学术期刊Renewable Energy (Q1，IF 9)发表了一篇关于液体脂肪酶催化高酸值油制备生物柴油的研究性论文。
脂肪酶直接催化美洲大蠊油（酸值为38.32 mg KOH/g）一步法制备生物柴油，而无需传统碱工艺的酸催化预处理步骤。在无溶剂体系中，脂肪酶Eversa® Transform 2.0（ET2）（12美元/千克）催化美洲大蠊油同时进行酯化和酯交换反应制备生物柴油。在最优反应条件下（反应温度32 °C、加酶量8.5 wt.%、反应时间8 h、醇/油摩尔比6.5/1、加水量4 wt.%），生物柴油的产率为98.63%。该酶回收6次，生物柴油产率仍高达 > 89.52%。在1 L反应器中，生物柴油产率仍可达93.94%在15.08 g/kg酶/生物柴油条件下。生物柴油成本估算为589.3美元/吨。动力学研究表明该反应的活化能为24.50 kJ/mol，动力学米氏常数为1.19 mol/L。生物柴油的理化指标符合中国和美国ASTM的标准，可与柴油混合并在两国应用。该研究表明脂肪酶可以在低温条件下直接催化废弃油脂制备生物柴油。
结果发表在Renewable Energy:
JJ Guo, S Gao, J Yang, H Zhang, YT Wang*, WN Ding*, Zhen Fang*. Biodiesel production via simultaneous esterification and transesterification of Periplaneta americana oil with liquid lipase Eversa® transform 2.0. Renewable Energy (IF 9), 229 (2024), 120756. https://doi.org/10.1016/j.renene.2024.120756
