Dual lipases immobilized on magnetic hydrophobic biochar for one-step production of biodiesel in deep eutectic solvent
Recently, PhD student Miss Jing-jing Guo supervised by Prof. Zhen Fang published a research article in Fuel about biodiesel production from high acid value oil with immobilized dual lipases.
Magnetic hydrophobic biochar was synthesized via pyrolysis of the chelated carboxymethyl cellulose/Fe3+ and modification by composite silanes, and subsequently used to covalently/physically immobilize dual lipases CALB/ET. The activity of dual lipases immobilized on hydrophobic magnetic biochar (16.1 U/mg) was increased by 11.8% than magnetic biochar, or 3.9% and 8.1% higher than that of single immobilized lipase ET (15.5 U/mg) and immobilized lipase CALB (14.9 U/mg), respectively. The highest loading of 39.5 mg lipase/g support was obtained with 17.9 U/mg activity. The immobilized dual lipases were stable with relative activity of 92.6% and 95.4% at 50 °C and pH 9 vs. 74.6% and 83.9% for liquid dual lipases, respectively. The immobilized dual lipases were further used for one-step production of biodiesel from acid oil (acid value 38 mg KOH/g) in deep eutectic solvent at 97.9% yield with 5 cycles (75.2%). Enzymatic reactions promote biodiesel purification, and crude biodiesel had 0.27 mg KOH/g acid value and 0.015% glycerol that met both Chinese national and US ASTM standards. The activation energy was 41.2 kJ/mol with kinetic Michaelis constant of 7.92×10-1 mol/L. The immobilized dual lipases for one-step production and purification of biodiesel presented excellent activity that provided a new insight for the development of novel immobilized lipase in biodiesel production.
Related results were published in Fuel:
Jing-jing Guo, Li Wang, Yi-tong Wang, Wo-na Ding, Wei-wei Liu, Zhen Fang*. Dual lipases immobilized on magnetic hydrophobic biochar for one-step production of biodiesel in deep eutectic solvent. Fuel, 381 (2025), 133497. https://doi.org/10.1016/j.fuel.2024.133497
Two lipases were successfully covalent/physical immobilized on magnetic hydrophobic biochar for direct production of biodiesel at 97.9% yield from acid oil at low temperature of 35 °C. （双脂肪酶成功共价/物理固定化在磁性疏水生物炭上并用于在35 °C低温下催化酸性油制备生物柴油，产率为97.9%）。
磁性疏水生物炭固定化双脂肪酶在低共熔溶剂中一步法生产生物柴油
近期，博士生郭静静同学在方真教授的指导下，在国际学术期刊Fuel（Q1，IF 6.7）发表了一篇关于固定化双脂肪酶催化高酸值油制备生物柴油的研究性论文。
通过热解羧甲基纤维素/Fe3+螯合物和复合硅烷的改性制备了磁性疏水生物炭，并用于共价/物理固定化双脂肪酶CALB/ET。双脂肪酶固定化在疏水磁性生物炭上的活性（16.1 U/mg）比其固定化在磁性生物炭上提高了11.8%。固定化双脂肪酶CALB/ET的活性比固定化脂肪酶ET（15.5 U/mg）和固定化脂肪酶CALB（14.9 U/mg）分别提高了3.9%和8.1%。最高酶载量为39.5 mg脂肪酶/g载体，酶活为17.9 U/mg。在50 °C和pH 9时，固定化双脂肪酶的相对酶活分别为92.6%和95.4%，而液体双脂肪酶的相对酶活为74.6%和83.9%。在低共熔溶剂体系中，固定化双脂肪酶用于催化酸性油（酸值38 mg KOH/g）一步法制备生物柴油，产率为97.9%。固定化双脂肪酶循环5次，生物柴油产率仍为75.2%。该反应体系促进了生物柴油的纯化，粗生物柴油的酸值为0.27 mg KOH/g，甘油含量为0.015%，符合中国和美国ASTM的标准。动力学研究表明该反应的活化能为41.2 kJ/mol，动力学米氏常数为7.92×10-1 mol/L。固定化双脂肪酶在一步法生产和纯化生物柴油中表现出优异的活性，为生物柴油生产中新型固定化脂肪酶的开发提供了新的见解。
结果发表在Fuel:
Jing-jing Guo, Li Wang, Yi-tong Wang, Wo-na Ding, Wei-wei Liu, Zhen Fang*. Dual lipases immobilized on magnetic hydrophobic biochar for one-step production of biodiesel in deep eutectic solvent. Fuel (IF 6.7), 381 (2025), 133497. https://doi.org/10.1016/j.fuel.2024.133497
